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  • Program agenda 2012
    gbbhotels com Programme day 1 Introduction to ENC Network BrainTrain Cerebnet SyMBaD Blitz presentations projects participating networks Transferable skills lectures Programme day 2 Selected oral reports participating networks Poster market and award students are informed who will contribute to the poster market Transferable skills lectures Keynote lecture Prof dr Guus Smit More information Days 2 Participants 50 Trieste 1 5 October 2012 Molecular basis of Neurodegeneration Content Neurogenomics This course will provide an overview of all the techniques involved in the study of gene expression in the brain including cell labelling cell purification RNA amplification microarray and deep sequencing Content Molecular basis of Neurodegeneration This conference will discuss the recent advances in neurodegenerative diseases with special emphasis on the mechanisms of cell type specific susceptibility and gene expression profiles experiments in post mortem brains as well as in mouse models of the disease Days 5 Participants 65 including 25 researchers Trieste 5 October 2012 Annual Meeting Magdeburg 12 18 December 2012 Finding molecular keys to brain dysfunction Content Bottom up or Top down how to find molecular keys to brain dysfunction Day 1 lectures concerning Molecules Synapses Networks Basic Principles of Neural Organization Following days Bottom up approaches like mouse mutants for synaptic proteins and Top down techniques like proteomics of the dysfunctional brain or Imaging genetics how genes influence brain structure or behaviour in animals or humans More information Days 5 Participants 50 including 18 researchers Heidelberg 19 20 December 2012 Supraresolution imaging of cells and tissues Content Topic is targeted perturbations of synaptic function using viral gene transfer and mouse genetics This includes methods of perturbations KO RNAi toxins dominant negatives silencers and actuators viral gene transfer systems stereotaxic approaches techniques of functional exploration of perturbations techniques for detection of structural alterations quantitative image processing determination of gene expression profiles and compensatory changes caused by perturbations modeling and simulation Practical didactic lectures will alternate with research talks presenting latest results using these technologies Practical demonstrations are organized in the laboratory if the total number of participants permits Arrival on 18 December from Magdeburg departure 20 December Mo 9 00 to 17 00 Lectures 18 30 Dinner Tue 9 00 to 12 00 Lectures 13 00 to 17 00 Practical demonstrations Workload 15h 0 6 ECTS Heidelberg 21 23 December 2012 Targeted molecular perturbation course Perturbation of synaptic function is designed as a practical course for a maximum of 6 students workload of 40h with a focus on stereotaxic methods anesthesia surgery cartesian coordinate system atlases validation of target area and viral gene transfer AAV production and purification Schedule Methods of perturbations KO RNAi toxins dominant negatives silencers and actuators Viral gene transfer systems Stereotaxic approaches Structural and functional exploration of perturbations Practical part learn how to Anesthesize rodents Perform stereotaxic surgery Stereotaxically deliver reagents into the brain Evaluate stereotaxic injection sites Design stereotaxic delivery experiment Wed 9 00 to 12 00 Lectures 14 00 to 18 00 practicals Thu 9 00 to 18 00 practicals Fri 9 00

    Original URL path: http://www.brain-train.nl/training-2/program-agenda-2012/ (2016-04-29)
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  • Program agenda 2013
    basic experimental principles and experimental design strategies for CAGE and discuss the use of high throughput sequencing in these experiments The interpretation of high throughput sequencing data is a challenging task that requires the use of bioinformatic tools We will outline commonly used bioinformatic tools methodologies and pipelines that allow us to study sequencing data Lastly we will provide examples of the use of such technologies in answering biological questions Course Schedule Yokohama City 19 September 2013 Neuroscience BrainTrain Conference See Program BrainTrain Conference Yokohama Japan For more information see www clst riken jp event 2013 BrainTrain html Download BrainTrain Conference Schedule Yokahama City 20 September 2013 Annual Meeting Paris April 2013 Electrophysiolical recording and imaging The first two days will be lectures Day 3 6 practical experiments with electrophysiological recording and imaging and demonstrations in local labs The practical experiments Extracellular spike recording and analysis Patch clamp recording and stimulation in slices CCD imaging of nerve tract excitation and postsynaptic recording Voltage sensitive indicators of membrane potential Photolysis Whole cell recording and CCD imaging of Ca concentration Dendritic patch clamp recording Perforated patch recording Leuven April 2013 mRNP transport in neurons mRNP transport in neurons and synaptic plasticity 15 persons Day 1 Morning 3 lectures on RNA protein transport and synaptic plasticity Afternoon Preparation of synaptosomes Day 2 and 3 Analysis of synaptic RNAs or proteins to be decided Training Program agenda 2011 Program agenda 2012 Program agenda 2013 Course Schedule Program BrainTrain Conference Techniques Events Training students can apply for the oncoming courses and workshops Read more Video JoVE video paper JoVE the Journal of Visualized Experiments gives the opportunity to share a method with the science community by actually showing how to prepare and perform an experiment We used the opportunity to create a video methods paper

    Original URL path: http://www.brain-train.nl/training-2/program-agenda-2013/ (2016-04-29)
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  • Course Schedule
    RNA sequencing Hiroshi Tarui 11 00 11 30 Coffee break 11 30 12 00 Applications of RNA sequencing Hiroshi Tarui 12 00 13 00 Lunch 13 00 14 30 Cap Analysis Gene Expression Masayoshi Itoh 14 30 15 00 Coffee break 15 00 15 30 Cap Analysis Gene Expression Masayoshi Itoh 15 30 17 00 Single molecule sequencing Sotaro Uemura 17 00 18 00 Additional lecture 2013 09 17 Day 2 Bioinformatic analyses 09 00 10 30 Promoter identification and network building Timo Lassmann 10 30 11 00 High throughput sequencing data analysis Hideya Kawaji 11 00 11 30 Coffee break 11 30 12 30 High throughput sequencing data analysis Hideya Kawaji 12 30 13 30 Lunch 13 30 14 30 Genome browsers Jessica Severin TBA 14 30 15 00 Coffee break 15 00 16 00 Data analysis using R Dave Tang 16 00 18 00 Hands on example of working with CAGE data Dave Tang 2013 09 18 Day 3 Applications of CAGE 9 00 10 30 Regulatory networks using CAGE Michiel de Hoon 10 30 11 00 Coffee break 11 00 12 00 nanoCAGE Charles Plessy 12 00 13 00 Lunch 13 00 14 30 FANTOM5 Alistair Forrest

    Original URL path: http://www.brain-train.nl/training-2/program-agenda-2013/course-schedule/ (2016-04-29)
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  • Program BrainTrain Conference
    Research Vrije Universiteit Amsterdam the Netherlands 10 00 11 00 Non invasive Prenatal Diagnosis of Genetic Disorders Professor Philippos Patsalis Chief Executive Medical Director Cyprus Institute of Neurology Genetics Chief Executive Director Cyprus School of Molecular Medicine Nicosia Cyprus 11 00 11 30 Coffee break 11 30 12 30 The effects of amyloid beta on synapses Dr Helmut Kessels Department Head Synaptic Plasticity and Behaviour Netherlands Institute for Neuroscience Amsterdam the Netherlands 12 30 14 00 Lunch 14 00 15 00 Probing Rules for Disturbing Synaptic Strength in Simple Networks Professor Yukiko Goda Senior Team Leader Neural Circuit Function RIKEN Brain Science Institute Tokyo Japan 15 00 16 00 Naturalistic Fearful Environment and Risky Decisions in Rats Professor Jeansok Kim Department of Psychology University of Washington Seattle USA 16 00 16 30 Coffee break 16 30 17 30 Development of Tafamidis a Transthyretin Stabilizer for Familial Amyloid Polyneuropathy Dr Yasuo Ochi and Dr Katsuyuki Machii Pfizer Japan 17 30 18 00 Upcoming Research Funding Opportunities Horizon 2020 Overview Dr Barbara Rhode Head of Science Technology Section Minister Counselor Delegation of the European Union to Japan Pfizer Japan 18 00 18 30 Closing Remarks Borrel 18 30 20 00 Dinner at

    Original URL path: http://www.brain-train.nl/training-2/program-agenda-2013/program-braintrai-conference/ (2016-04-29)
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  • Techniques
    in all the research teams Neuronal culture Organotypic culture slices Primary culture neurons glia Trans Infection siRNA technology Molecular biology Generation viral mediated expression constructs Stereotaxic injections viral gene transfer Generation of epitope tagged proteins Purification of tagged fusion proteins Site directed mutagenesis Large scale automated sequencing Generation of knock out transgenic mice mRNA RNA translational assays mRNA RNA stability assays Gene Protein profiling Real time quantitative PCR Construction expression libraries Massive Parralel Sequencing Data analysis Mass spectrometry LC LC MS MS Protein chemistry Electrophoresis 1D 2D PAGE Immunoprecipitation Western blot Postsynaptic membrane fractionation Surface Plasmon Resonance Protein phosphorylation assays Neural imaging Evanescent Wave Microscopy Immunofluorescence Digital Electron Microscopy Morphometry 2 photon microscopy Spot confocal microscopy High temporal resolution confocal microscopy Brain genetics STR and SNP genotype analysis Positional cloning LD analysis Quantitative genome analysis MLPA Clinical diagnosis neuropathology linkage association analysis statistics Electro physiology Current and voltage patch clamping Functional imaging photodiode array Multi array recordings ECG monitoring Computer science Modelling of neural networks and protein complexes Quantal analysis mathematical modelling Development of new non linear algorithms for behavioural phenotyping Behaviour High Low throughput phenotyping Autonomic readouts Training Program agenda 2011 Program agenda 2012 Program agenda 2013 Course Schedule Program BrainTrain Conference Techniques Events Training students can apply for the oncoming courses and workshops Read more Video JoVE video paper JoVE the Journal of Visualized Experiments gives the opportunity to share a method with the science community by actually showing how to prepare and perform an experiment We used the opportunity to create a video methods paper of the well established calcium imaging technique in immature brain slices Read more brain consortium brain diseases brain disorders brain dysfunction brain research BrainTrain dementia depressive disorder diseased brain Early Stage Researcher ESR EUsynapse gene mapping genetic approach Genetics of the brain

    Original URL path: http://www.brain-train.nl/training-2/techniques/ (2016-04-29)
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  • General public
    function and orchestrate the complexity of neuronal circuitry and that ultimately translate into complex abberant behavior Working at different levels of organization molecular network or whole organism individual members of the BrainTrain consortium have recently made important progress in unravelling the principles of neuronal complexity Members are also engaged in the description of gene regulatory and transcriptional network within larger neuronal networks Partners have discovered novel principles of how neurons pass information onto other neurons and how to share transmitter vesicles among synapses Gene cascades that modulate synaptic transmission have been characterized and how modulation of individual synapses affects network properties has been unravelled Loci and genes for several major brain disorders have been discovered by BrainTrain participants and novel strategies to identify genetic variations that contribute to complex brain disorders have been designed In addition well characterized patient populations cohorts have been collected within the consortium Events Training students can apply for the oncoming courses and workshops Read more Video JoVE video paper JoVE the Journal of Visualized Experiments gives the opportunity to share a method with the science community by actually showing how to prepare and perform an experiment We used the opportunity to create a video methods

    Original URL path: http://www.brain-train.nl/general-public/ (2016-04-29)
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  • Brain-Train | | Page 2
    the perisynaptic ECM In particular the role of ECM modifying and degrading enzymes such as hyaluronidases and matrix metalloproteinases in plasticity processes and in pathology of the adult central nervous system will be studied This partner will provide know how in glycobiology and biochemistry of the ECM as well as imaging of local cell surface processes including single molecule tracking and two color STED microscopy Posted in partners Comments closed Institut Pasteur IP By karen Published September 15 2010 Centre National de la Recherche Scientifique CNRS Laboratory of Integrative Neurobiology of Cholinergic Systems Paris France Uwe Maskos Our laboratory focuses on a couple of major issues in public health 100 million people are expected to die this century from the consequences of nicotine addiction yet nicotine is also known to enhance cognitive performance There is clear cut epidemiological evidence in the medical literature confirmed by meta analyses that smoking protects against Parkinson s Disease PD and also Alzheimer s Disease AD Additionally nicotinic acetylcholine receptors nAChRs are down regulated in serious human psychiatric conditions like autism Hence the identification of the molecular mechanisms and circuits involved in nicotine reinforcement nicotine mediated neuroprotection and cognition is urgent and requires the development of novel tools that allow genetic and molecular manipulation in vivo in experimental animals Posted in partners Comments closed Sylics SYL By karen Published September 15 2010 Sylics the Netherlands Amsterdam Rhiannon Meredith The project partner and Early Stage Researcher will work within Sylics tradename of Synaptologics WP 4 to characterise the spatial and temporal patterns of synaptic activity and plasticity mechanisms underlying development of cortical and hippocampal neuronal circuits In inherited neurodevelopmental disorders of autism and mental retardation the genetic effects on the development and maturation of brain activity are not known We will use electrophysiology and imaging techniques to establish whether synaptic network function is altered during early brain development in genetic mouse models of autism and mental retardation Posted in partners Comments closed Karolinska Institutet By karen Published September 15 2010 Department of Physiology and Pharmacology Stockholm Sweden Per Svenningson The group will contribute with its expertise in study of intracellular signaling in vivo and in vitro The work will include in vivo studies of actions via GPCRs adaptor proteins and protein phosphorylation pathways in response to pharmacological and behavioural stimulation in wild type and mutant mice An emphasis will be put on serotonin mediated modulation of glutamatergic transmission The group will closely interact with other partners and provide data for modeling Posted in partners Comments closed VU University Medical Center Amsterdam VUmc By karen Published September 15 2010 Department of Functional Genomics the Netherlands Amsterdam Heidi de Wit Many brain disorders e g mental retardation attention deficits are considered synaptopathies which result from alterations in the molecular and cellular function of synapses To visualize this disease linked synaptic alterations at nanometer level electron microscopical EM methods are required which is exactly the main task of this project partner Using EM techniques the research team of De

    Original URL path: http://www.brain-train.nl/training-2/weblogs/page/2/ (2016-04-29)
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  • The finale
    think there are many programs out there that can rival what I ve been part of I say this with respect to the networking opportunities I ve been given the unique experience of being able take part in workshops and visit prestigious research institutes around Europe and the opportunity to work with world class scientists from around the world Heck I could also work in Italy for 2 months I ve learned so much in these three years and benefited from the interdisciplinary nature of BrainTrain i e from genes to behaviour Sure there were the innate problems that plague interdisciplinary consortia and interdisciplinary sciences but the advantages we could benefit from far outweighed the disadvantages I ve built good friendships with my colleagues in BrainTrain and while our final meeting is looming I know that this is in no way the end of our relationship And I m sure in the future we will bump into each other at meetings conferences or the local pub and remind each other of the fond memories we had during our time with BrainTrain But for now see you guys and gals very soon in Japan Dave This entry was posted in weblog Bookmark the permalink Post a comment or leave a trackback Trackback URL Post a Comment Click here to cancel reply Your email is never published nor shared Required fields are marked Name Email Website Comment You may use these HTML tags and attributes a href title abbr title acronym title b blockquote cite cite code del datetime em i q cite s strike strong s en 15 Events Training students can apply for the oncoming courses and workshops Read more Video JoVE video paper JoVE the Journal of Visualized Experiments gives the opportunity to share a method with the science

    Original URL path: http://www.brain-train.nl/blog/2013/09/06/the-finale/ (2016-04-29)
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