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  • Erasmus MC : Research
    postmeiotische regulatie van chromatine DNA dubbelstrengsbreuken chromosoomparing en transcriptionele inactivatie tijdens de meiotische profase Links Biomedische Wetenschappen Biomedische Wetenschappen intranet medewerkers Intranet Ontwikkelingbiologie alleen medewerkers Medische Bibliotheek Optical Imaging Centre International Society of Andrology Transcriptional Control in Developmental Processes Linker Menu Introductie Agenda Research X chromosoom inactivatie Meiotische en postmeiotische regulatie van chromatine Onderwijs Employees Contact 010 704 0 704 Uw reactie Over deze site Disclaimer Privacy Cookies 2015 Erasmus

    Original URL path: http://www.erasmusmc.nl/rede/research/ (2015-12-02)
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  • Internal Link
    Route lang search Uitgebreid zoeken Erasmus MC International office Patiëntenzorg Go Research Go Onderwijs Go Over Erasmus MC Go Werken bij Go Patiëntenzorg Research Onderwijs Over Erasmus MC Werken bij Medewerkers Mijn Erasmus MC Contact en Route Faciliteiten International office

    Original URL path: http://www.erasmusmc.nl/research/faciliteiten/4059930/ (2015-12-02)
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  • Erasmus MC : Contact Proteomics Center
    erasmusmc nl Would you like to start a collaborative project Contact us for more information Website www proteomicscenter nl Dr Jeroen Demmers Postadres Proteomics Center Erasmus MC Office Ee 679A Lab Ee 626 Wytemaweg 80 3015 CN Rotterdam Linker Menu Introduction proteomicscenter nl Background Research Equipment Lab members Publications 010 704 0 704 Uw reactie Over deze site Disclaimer Privacy Cookies 2015 Erasmus MC Deze site maakt gebruik van cookies

    Original URL path: http://www.erasmusmc.nl/proteomics/4517291/ (2015-12-02)
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  • Erasmus MC Proteomics Center: Introduction
    Netherlands and outside Research projects in our laboratory focus on the characterization of protein complexes interactomics the analysis of protein posttranslational modifications in relation to their biochemical roles and on the analysis of sub cellular proteomes and the dynamics thereof The key technology in our lab is quantitative mass spectrometry nanoLC MS MS based proteomics SILAC TMT iTRAQ 18O dimethylation and label free approaches The ultimate goal of our research is to develop analytical tools to better understand how cellular processes are controlled at the molecular level The Center closely collaborates with many research departments at Erasmus MC and several research groups within The Netherlands and abroad Much of this work was and is done within the context of national and international research schools and consortia such as the Cancer Genomics Center the Center for Biomedical Genetics the Medisch Genetisch Centrum Epigenome NoE Eurythron the Netherlands Proteomics Center EuTRACC and Proteins Work Zie ook Nieuwsarchief Persberichten Mail de redactie Print Linker Menu Introduction proteomicscenter nl Background Research Equipment Lab members Publications 010 704 0 704 Uw reactie Over deze site Disclaimer Privacy Cookies 2015 Erasmus MC Deze site maakt gebruik van cookies Lees meer Gezien Help mee onze website te

    Original URL path: http://www.erasmusmc.nl/proteomics/introduction/ (2015-12-02)
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  • Erasmus MC Proteomics Center: Research
    by changing the chemical nature of an amino acid or by making structural changes like the formation of disulfide bridges We try to answer questions such as how amino acid modifications can affect protein complex composition and how they are involved in cellular functioning Mass spectrometry has proven to be an excellent tool for the characterization of posttranslational modifications such as acetylation methylation phosphorylation ubiquitylation ISGylation and so on In order to analyze such modifications by mass spectrometry it is often necessary to enrich for proteolytic peptides bearing the modification of interest For example phosphorylated peptides can be enriched for using a protocol that involves trapping such peptides on TiO2 chromatographic column material Furthermore we are developing tools for the quantitative analysis of dynamic posttranslational modifications and try to answer questions such as how the phosphorylation state of a protein complex depends on its position in the cell cycle and how modifications on histones and nucleosome associated proteins change after DNA damage Recently we have characterized by mass spectrometry multiple sites of ubiquitylation on Tramtrack a protein which was found to be the major substrate of the ubiquitin specific protease UBP64 in Drosophila link to MCB paper 18 O and SILAC labeling for quantitative proteomics We have optimized and implemented a method that uses 18 O labeling of tryptic peptides to account for quantitative differences in protein levels between different samples When all proteolytic peptides in e g a control sample are labeled with 18 O we can easily differentiate between real interactors of our protein of interest and non specific background proteins in the case of background proteins both heavily labeled and non labeled peptides will appear in the same mass spectrum In contrast if the protein is specific for the sample only the light variant will be observed We have successfully applied this method for quickly subtracting background proteins from real interactors in co immunoprecipitations Another way to study changes in the proteome in a quantitative manner is by using Stable Isotope Labeling of Amino acids in Cell culture SILAC In this approach one sample originates from cells that were grown under normal conditions and the other sample is from cells that were grown in media containing amino acids that were labeled with heavy carbon 13 C nitrogen 15 N and or hydrogen D isotopes One of our reserach projects involves investigation of changes in histone modifications after DNA damage and we use SILAC to identify and quantitate such changes Characterization of complex protein mixtures For highly complex mixtures like complete cellular lysates nuclear extracts or body fluids multi dimensional separations of proteins and or proteolytic peptides should be applied to get the highest number of protein identifications Before reversed phase separation on a C18 column in the typical nanoflow LC MS MS setup that we use in the lab peptides can be separated and fractionated by either strong cation exchange SCX strong anion exchange SAX or iso electric focusing IEF Usually 10 to 20 fractions for each

    Original URL path: http://www.erasmusmc.nl/proteomics/research/ (2015-12-02)
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  • Erasmus MC Proteomics Center: Publications
    Mar 7 PubMed PMID 23500724 15 Raj VS Mou H Smits SL Dekkers DH Müller MA Dijkman R Muth D Demmers JA Zaki A Fouchier RA Thiel V Drosten C Rottier PJ Osterhaus AD Bosch BJ Haagmans BL Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus EMC Nature 2013 Mar 14 495 7440 251 4 doi 10 1038 nature12005 PubMed PMID 23486063 16 Kuster DW Sequeira V Najafi A Boontje NM Wijnker PJ Witjas Paalberends ER Marston SB Dos Remedios CG Carrier L Demmers JA Redwood C Sadayappan S van der Velden J GSK3β phosphorylates newly identified site in the proline alanine rich region of cardiac myosin binding protein C and alters cross bridge cycling kinetics in human short communication Circ Res 2013 Feb 15 112 4 633 9 doi 10 1161 CIRCRESAHA 112 275602 Epub 2012 Dec 31 PubMed PMID 23277198 PubMed Central PMCID PMC3595322 17 van Spronsen M Mikhaylova M Lipka J Schlager MA van den Heuvel DJ Kuijpers M Wulf PS Keijzer N Demmers J Kapitein LC Jaarsma D Gerritsen HC Akhmanova A Hoogenraad CC TRAK Milton motor adaptor proteins steer mitochondrial trafficking to axons and dendrites Neuron 2013 Feb 6 77 3 485 502 doi 10 1016 j neuron 2012 11 027 PubMed PMID 23395375 18 Moshkin YM Doyen CM Kan TW Chalkley GE Sap K Bezstarosti K Demmers JA Ozgur Z van Ijcken WF Verrijzer CP Histone chaperone NAP1 mediates sister chromatid resolution by counteracting protein phosphatase 2A PLoS Genet 2013 9 9 e1003719 doi 10 1371 journal pgen 1003719 Epub 2013 Sep 26 PubMed PMID 24086141 PubMed Central PMCID PMC3784504 19 Splinter D Razafsky DS Schlager MA Serra Marques A Grigoriev I Demmers J Keijzer N Jiang K Poser I Hyman AA Hoogenraad CC King SJ Akhmanova A BICD2 dynactin and LIS1 cooperate in regulating dynein recruitment to cellular structures Mol Biol Cell 2012 Nov 23 21 4226 41 doi 10 1091 mbc E12 03 0210 Epub 2012 Sep 5 PubMed PMID 22956769 PubMed Central PMCID PMC3484101 20 Fanis P Gillemans N Aghajanirefah A Pourfarzad F Demmers J Esteghamat F Vadlamudi RK Grosveld F Philipsen S van Dijk TB Five friends of methylated chromatin target of protein arginine methyltransferase prmt 1 chtop a complex linking arginine methylation to desumoylation Mol Cell Proteomics 2012 Nov 11 11 1263 73 doi 10 1074 mcp M112 017194 Epub 2012 Aug 7 PubMed PMID 22872859 PubMed Central PMCID PMC3494204 21 Talens S Lebbink JH Malfliet JJ Demmers JA Uitte de Willige S Leebeek FW Rijken DC Binding of carboxypeptidase N to fibrinogen and fibrin Biochem Biophys Res Commun 2012 Oct 19 427 2 421 5 doi 10 1016 j bbrc 2012 09 081 Epub 2012 Sep 21 PubMed PMID 23000409 22 Jiang K Toedt G Montenegro Gouveia S Davey NE Hua S van der Vaart B Grigoriev I Larsen J Pedersen LB Bezstarosti K Lince Faria M Demmers J Steinmetz MO Gibson TJ Akhmanova A A Proteome wide screen for mammalian SxIP motif containing microtubule plus end tracking proteins Curr Biol 2012 Oct 9 22 19 1800 7 doi 10 1016 j cub 2012 07 047 Epub 2012 Aug 9 PubMed PMID 22885064 23 van Riel B Pakozdi T Brouwer R Monteiro R Tuladhar K Franke V Bryne JC Jorna R Rijkers EJ van Ijcken W Andrieu Soler C Demmers J Patient R Soler E Lenhard B Grosveld F A novel complex RUNX1 MYEF2 represses hematopoietic genes in erythroid cells Mol Cell Biol 2012 Oct 32 19 3814 22 doi 10 1128 MCB 05938 11 Epub 2012 Jul 16 PubMed PMID 22801375 PubMed Central PMCID PMC3457535 24 de Graaff E Maat P Hulsenboom E van den Berg R van den Bent M Demmers J Lugtenburg PJ Hoogenraad CC Sillevis Smitt P Identification of delta notch like epidermal growth factor related receptor as the Tr antigen in paraneoplastic cerebellar degeneration Ann Neurol 2012 Jun 71 6 815 24 doi 10 1002 ana 23550 Epub 2012 Mar 23 PubMed PMID 22447725 25 Mohd Sarip A Lagarou A Doyen CM van der Knaap JA Aslan Ü Bezstarosti K Yassin Y Brock HW Demmers JA Verrijzer CP Transcription independent function of Polycomb group protein PSC in cell cycle control Science 2012 May 11 336 6082 744 7 doi 10 1126 science 1215927 Epub 2012 Apr 5 PubMed PMID 22491092 26 Schwertman P Lagarou A Dekkers DH Raams A van der Hoek AC Laffeber C Hoeijmakers JH Demmers JA Fousteri M Vermeulen W Marteijn JA UV sensitive syndrome protein UVSSA recruits USP7 to regulate transcription coupled repair Nat Genet 2012 May 44 5 598 602 doi 10 1038 ng 2230 PubMed PMID 22466611 27 Twigt J Steegers Theunissen RP Bezstarosti K Demmers JA Proteomic analysis of the microenvironment of developing oocytes Proteomics 2012 May 12 9 1463 71 doi 10 1002 pmic 201100240 PubMed PMID 22589193 28 Gontan C Achame EM Demmers J Barakat TS Rentmeester E van IJcken W Grootegoed JA Gribnau J RNF12 initiates X chromosome inactivation by targeting REX1 for degradation Nature 2012 Apr 29 485 7398 386 90 doi 10 1038 nature11070 PubMed PMID 22596162 29 Brazão TF Demmers J van IJcken W Strouboulis J Fornerod M Romão L Grosveld FG A new function of ROD1 in nonsense mediated mRNA decay FEBS Lett 2012 Apr 24 586 8 1101 10 doi 10 1016 j febslet 2012 03 015 Epub 2012 Mar 21 PubMed PMID 22575643 30 McDonel P Demmers J Tan DW Watt F Hendrich BD Sin3a is essential for the genome integrity and viability of pluripotent cells Dev Biol 2012 Mar 1 363 1 62 73 doi 10 1016 j ydbio 2011 12 019 Epub 2011 Dec 20 PubMed PMID 22206758 PubMed Central PMCID PMC3334623 31 Tavares L Dimitrova E Oxley D Webster J Poot R Demmers J Bezstarosti K Taylor S Ura H Koide H Wutz A Vidal M Elderkin S Brockdorff N RYBP PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3 Cell 2012 Feb 17 148 4 664 78 doi 10 1016 j cell 2011 12 029 Epub 2012 Feb 9 Erratum in Cell 2012 Jun 22 149 7 1647 8 PubMed PMID 22325148 PubMed Central PMCID PMC3281992 32 Moshkin YM Chalkley GE Kan TW Reddy BA Ozgur Z van Ijcken WF Dekkers DH Demmers JA Travers AA Verrijzer CP Remodelers organize cellular chromatin by counteracting intrinsic histone DNA sequence preferences in a class specific manner Mol Cell Biol 2012 Feb 32 3 675 88 doi 10 1128 MCB 06365 11 Epub 2011 Nov 28 PubMed PMID 22124157 PubMed Central PMCID PMC3266603 33 Talens S Leebeek FW Demmers JA Rijken DC Identification of fibrin clot bound plasma proteins PLoS One 2012 7 8 e41966 doi 10 1371 journal pone 0041966 Epub 2012 Aug 3 PubMed PMID 22870270 PubMed Central PMCID PMC3411686 34 Alves RD Demmers JA Bezstarosti K van der Eerden BC Verhaar JA Eijken M van Leeuwen JP Unraveling the human bone microenvironment beyond the classical extracellular matrix proteins a human bone protein library J Proteome Res 2011 Oct 7 10 10 4725 33 doi 10 1021 pr200522n Epub 2011 Sep 21 Erratum in J Proteome Res 2011 Dec 2 10 2 5576 PubMed PMID 21892838 35 Leotoing L Chereau F Baron S Hube F Valencia HJ Bordereaux D Demmers JA Strouboulis J Baud V A20 binding inhibitor of nuclear factor kappaB NF kappaB 2 ABIN 2 is an activator of inhibitor of NF kappaB IkappaB kinase alpha IKKalpha mediated NF kappaB transcriptional activity J Biol Chem 2011 Sep 16 286 37 32277 88 doi 10 1074 jbc M111 236448 Epub 2011 Jul 22 PubMed PMID 21784860 PubMed Central PMCID PMC3173178 36 Esteghamat F van Dijk TB Braun H Dekker S van der Linden R Hou J Fanis P Demmers J van IJcken W Ozgür Z Horos R Pourfarzad F von Lindern M Philipsen S The DNA binding factor Hmg20b is a repressor of erythroid differentiation Haematologica 2011 Sep 96 9 1252 60 doi 10 3324 haematol 2011 045211 Epub 2011 May 23 PubMed PMID 21606163 PubMed Central PMCID PMC3166094 37 Cui S Kolodziej KE Obara N Amaral Psarris A Demmers J Shi L Engel JD Grosveld F Strouboulis J Tanabe O Nuclear receptors TR2 and TR4 recruit multiple epigenetic transcriptional corepressors that associate specifically with the embryonic β type globin promoters in differentiated adult erythroid cells Mol Cell Biol 2011 Aug 31 16 3298 311 doi 10 1128 MCB 05310 11 Epub 2011 Jun 13 PubMed PMID 21670149 PubMed Central PMCID PMC3147791 38 Meenhuis A van Veelen PA de Looper H van Boxtel N van den Berge IJ Sun SM Taskesen E Stern P de Ru AH van Adrichem AJ Demmers J Jongen Lavrencic M Löwenberg B Touw IP Sharp PA Erkeland SJ MiR 17 20 93 106 promote hematopoietic cell expansion by targeting sequestosome 1 regulated pathways in mice Blood 2011 Jul 28 118 4 916 25 doi 10 1182 blood 2011 02 336487 Epub 2011 May 31 PubMed PMID 21628417 PubMed Central PMCID PMC3148171 39 van der Vaart B Manatschal C Grigoriev I Olieric V Gouveia SM Bjelic S Demmers J Vorobjev I Hoogenraad CC Steinmetz MO Akhmanova A SLAIN2 links microtubule plus end tracking proteins and controls microtubule growth in interphase J Cell Biol 2011 Jun 13 193 6 1083 99 doi 10 1083 jcb 201012179 Epub 2011 Jun 6 PubMed PMID 21646404 PubMed Central PMCID PMC3115796 40 Grigoriev I Yu KL Martinez Sanchez E Serra Marques A Smal I Meijering E Demmers J Peränen J Pasterkamp RJ van der Sluijs P Hoogenraad CC Akhmanova A Rab6 Rab8 and MICAL3 cooperate in controlling docking and fusion of exocytotic carriers Curr Biol 2011 Jun 7 21 11 967 74 doi 10 1016 j cub 2011 04 030 Epub 2011 May 19 PubMed PMID 21596566 41 Kapitein LC Yau KW Gouveia SM van der Zwan WA Wulf PS Keijzer N Demmers J Jaworski J Akhmanova A Hoogenraad CC NMDA receptor activation suppresses microtubule growth and spine entry J Neurosci 2011 Jun 1 31 22 8194 209 doi 10 1523 JNEUROSCI 6215 10 2011 PubMed PMID 21632941 42 Engelen E Akinci U Bryne JC Hou J Gontan C Moen M Szumska D Kockx C van Ijcken W Dekkers DH Demmers J Rijkers EJ Bhattacharya S Philipsen S Pevny LH Grosveld FG Rottier RJ Lenhard B Poot RA Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes Nat Genet 2011 Jun 43 6 607 11 doi 10 1038 ng 825 Epub 2011 May 1 PubMed PMID 21532573 43 Casanova M Preissner T Cerase A Poot R Yamada D Li X Appanah R Bezstarosti K Demmers J Koseki H Brockdorff N Polycomblike 2 facilitates the recruitment of PRC2 Polycomb group complexes to the inactive X chromosome and to target loci in embryonic stem cells Development 2011 Apr 138 8 1471 82 doi 10 1242 dev 053652 Epub 2011 Mar 2 PubMed PMID 21367819 PubMed Central PMCID PMC3062419 44 Soler E Andrieu Soler C Boer Ed Bryne JC Thongjuea S Rijkers E Demmers J Ijcken Wv Grosveld F A systems approach to analyze transcription factors in mammalian cells Methods 2011 Feb 53 2 151 62 doi 10 1016 j ymeth 2010 08 002 Epub 2010 Aug 10 Review PubMed PMID 20705139 45 Domingues I Rino J Demmers JA de Lanerolle P Santos SC VEGFR2 translocates to the nucleus to regulate its own transcription PLoS One 2011 6 9 e25668 doi 10 1371 journal pone 0025668 Epub 2011 Sep 28 PubMed PMID 21980525 PubMed Central PMCID PMC3182252 46 van de Nobelen S Rosa Garrido M Leers J Heath H Soochit W Joosen L Jonkers I Demmers J van der Reijden M Torrano V Grosveld F Delgado MD Renkawitz R Galjart N Sleutels F CTCF regulates the local epigenetic state of ribosomal DNA repeats Epigenetics Chromatin 2010 Nov 8 3 1 19 doi 10 1186 1756 8935 3 19 PubMed PMID 21059229 PubMed Central PMCID PMC2993708 47 Reddy BA Bajpe PK Bassett A Moshkin YM Kozhevnikova E Bezstarosti K Demmers JA Travers AA Verrijzer CP Drosophila transcription factor Tramtrack69 binds MEP1 to recruit the chromatin remodeler NuRD Mol Cell Biol 2010 Nov 30 21 5234 44 doi 10 1128 MCB 00266 10 Epub 2010 Aug 23 PubMed PMID 20733004 PubMed Central PMCID PMC2953047 48 Kubben N Voncken JW Demmers J Calis C van Almen G Pinto Y Misteli T Identification of differential protein interactors of lamin A and progerin Nucleus 2010 Nov Dec 1 6 513 25 doi 10 4161 nucl 1 6 13512 Epub 2010 Sep 3 PubMed PMID 21327095 PubMed Central PMCID PMC3027055 49 Bezstarosti K Ghamari A Grosveld FG Demmers JA Differential proteomics based on 18O labeling to determine the cyclin dependent kinase 9 interactome J Proteome Res 2010 Sep 3 9 9 4464 75 doi 10 1021 pr100217d PubMed PMID 20593818 50 Krebs AR Demmers J Karmodiya K Chang NC Chang AC Tora L ATAC and Mediator coactivators form a stable complex and regulate a set of non coding RNA genes EMBO Rep 2010 Jul 11 7 541 7 doi 10 1038 embor 2010 75 Epub 2010 May 28 PubMed PMID 20508642 PubMed Central PMCID PMC2897115 51 Landeira D Sauer S Poot R Dvorkina M Mazzarella L Jørgensen HF Pereira CF Leleu M Piccolo FM Spivakov M Brookes E Pombo A Fisher C Skarnes WC Snoek T Bezstarosti K Demmers J Klose RJ Casanova M Tavares L Brockdorff N Merkenschlager M Fisher AG Jarid2 is a PRC2 component in embryonic stem cells required for multi lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators Nat Cell Biol 2010 Jun 12 6 618 24 doi 10 1038 ncb2065 Epub 2010 May 16 PubMed PMID 20473294 PubMed Central PMCID PMC3572404 52 Splinter D Tanenbaum ME Lindqvist A Jaarsma D Flotho A Yu KL Grigoriev I Engelsma D Haasdijk ED Keijzer N Demmers J Fornerod M Melchior F Hoogenraad CC Medema RH Akhmanova A Bicaudal D2 dynein and kinesin 1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry PLoS Biol 2010 Apr 6 8 4 e1000350 doi 10 1371 journal pbio 1000350 PubMed PMID 20386726 PubMed Central PMCID PMC2850381 53 van den Berg DL Snoek T Mullin NP Yates A Bezstarosti K Demmers J Chambers I Poot RA An Oct4 centered protein interaction network in embryonic stem cells Cell Stem Cell 2010 Apr 2 6 4 369 81 doi 10 1016 j stem 2010 02 014 PubMed PMID 20362541 PubMed Central PMCID PMC2860243 54 van Dijk TB Gillemans N Stein C Fanis P Demmers J van de Corput M Essers J Grosveld F Bauer UM Philipsen S Friend of Prmt1 a novel chromatin target of protein arginine methyltransferases Mol Cell Biol 2010 Jan 30 1 260 72 doi 10 1128 MCB 00645 09 Epub PubMed PMID 19858291 PubMed Central PMCID PMC2798285 55 Pilyugin M Demmers J Verrijzer CP Karch F Moshkin YM Phosphorylation mediated control of histone chaperone ASF1 levels by Tousled like kinases PLoS One 2009 Dec 16 4 12 e8328 doi 10 1371 journal pone 0008328 PubMed PMID 20016786 PubMed Central PMCID PMC2791443 56 Moshkin YM Kan TW Goodfellow H Bezstarosti K Maeda RK Pilyugin M Karch F Bray SJ Demmers JA Verrijzer CP Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID RPD3 complexes during NOTCH silencing Mol Cell 2009 Sep 24 35 6 782 93 doi 10 1016 j molcel 2009 07 020 Erratum in Mol Cell 2013 Jul 11 51 1 128 9 PubMed PMID 19782028 57 Niessen HE Demmers JA Voncken JW Talking to chromatin post translational modulation of polycomb group function Epigenetics Chromatin 2009 Sep 1 2 1 10 doi 10 1186 1756 8935 2 10 PubMed PMID 19723311 PubMed Central PMCID PMC2745409 58 Gontan C Güttler T Engelen E Demmers J Fornerod M Grosveld FG Tibboel D Görlich D Poot RA Rottier RJ Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors J Cell Biol 2009 Apr 6 185 1 27 34 doi 10 1083 jcb 200810106 PubMed PMID 19349578 PubMed Central PMCID PMC2700522 59 Wong HY Demmers JA Bezstarosti K Grootegoed JA Brinkmann AO DNA dependent recruitment of DDX17 and other interacting proteins by the human androgen receptor Biochim Biophys Acta 2009 Feb 1794 2 193 8 doi 10 1016 j bbapap 2008 11 001 Epub 2008 Nov 14 PubMed PMID 19059367 60 Jaworski J Kapitein LC Gouveia SM Dortland BR Wulf PS Grigoriev I Camera P Spangler SA Di Stefano P Demmers J Krugers H Defilippi P Akhmanova A Hoogenraad CC Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity Neuron 2009 Jan 15 61 1 85 100 doi 10 1016 j neuron 2008 11 013 PubMed PMID 19146815 61 Lagarou A Mohd Sarip A Moshkin YM Chalkley GE Bezstarosti K Demmers JA Verrijzer CP dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing Genes Dev 2008 Oct 15 22 20 2799 810 doi 10 1101 gad 484208 PubMed PMID 18923078 PubMed Central PMCID PMC2569881 62 van den Berg DL Zhang W Yates A Engelen E Takacs K Bezstarosti K Demmers J Chambers I Poot RA Estrogen related receptor beta interacts with Oct4 to positively regulate Nanog gene expression Mol Cell Biol 2008 Oct 28 19 5986 95 doi 10 1128 MCB 00301 08 Epub 2008 Jul 28 PubMed PMID 18662995 PubMed Central PMCID PMC2547019 63 Dekkers DH Bezstarosti K Gurusamy N Luijk K Verhoeven AJ Rijkers EJ Demmers JA Lamers JM Maulik N Das DK Identification by a differential proteomic approach of the induced stress and redox proteins by resveratrol in the normal and diabetic rat heart J Cell Mol Med 2008 Sep Oct 12 5A 1677 89 doi 10 1111 j 1582 4934 2008 00227 x Epub 2008 Jan 11 PubMed PMID 18194459 64 Chalkley GE Moshkin YM Langenberg K Bezstarosti K Blastyak A Gyurkovics H Demmers JA Verrijzer CP The transcriptional coactivator SAYP is a trithorax group signature

    Original URL path: http://www.erasmusmc.nl/proteomics/publications/ (2015-12-02)
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  • Erasmus MC Proteomics Center: Background
    larger complexes to exert their function and one of our major goals to identify which proteins interact with each other This often gives important clues about the functions of newly discovered proteins Several methods are available to probe such protein protein interactions most importantly immunoaffinity chromatography under mild conditions followed by mass spectrometry to identify the proteins Since proteins play a central role in the life of an organism proteomics is thought to be instrumental in finding novel biomarkers such as markers that indicate a particular disease Mass spectrometry A mass spectrometer determines the mass of a molecule by measuring the mass to charge ratio m z of its ion Ions are generated by inducing either the loss or gain of a charge from a neutral species Once formed ions are electrostatically directed into a mass analyzer where they are separated according to m z and finally detected The result of molecular ionization ion separation and ion detection is a spectrum that can provide molecular mass and even structural information Over the past decade mass spectrometry has undergone tremendous technological improvements allowing for its application to proteins peptides carbohydrates DNA drugs and many other biologically relevant molecules Due to ionization sources such as electrospray ionization ESI and matrix assisted laser desorption ionization MALDI mass spectrometry has become an irreplaceable tool in the biological sciences According to John B Fenn the inventor of electrospray ionization for biomolecules and the 2002 Nobel Laureate in Chemistry mass spectrometry is the art of measuring atoms and molecules to determine their molecular weight Such mass or weight information is sometimes sufficient frequently necessary and always useful in determining the identity of a species To practice this art one puts charge on the molecules of interest i e the analyte then measures how the trajectories of

    Original URL path: http://www.erasmusmc.nl/proteomics/background/ (2015-12-02)
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  • Erasmus MC Proteomics Center: Equipment
    LTQ Orbitrap Thermo plus ETD LTQ Orbitrap Thermo LTQ linear ion trap Thermo Q ToF Xevo Waters Triple Quad Xevo Waters NEW An Orbitrap Fusion Tribrid will be installed soon Zie ook Nieuwsarchief Persberichten Mail de redactie Print Linker Menu Introduction proteomicscenter nl Background Research Equipment Lab members Publications 010 704 0 704 Uw reactie Over deze site Disclaimer Privacy Cookies 2015 Erasmus MC Deze site maakt gebruik van cookies

    Original URL path: http://www.erasmusmc.nl/proteomics/equipment/ (2015-12-02)
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